Anti-Mouse CD117 Antibody, Clone ACK2_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD117 Antibody, Clone ACK2

Rat monoclonal IgG2b antibody against mouse CD117 (c-Kit)

概要

技术资料

数据及文献

概要

The ACK2 antibody reacts with CD117 (c-Kit), an ~145 kDa type 1 transmembrane receptor for c-Kit ligand (stem cell factor/steel factor) that is broadly expressed on hematopoietic stem cells in bone marrow, including pluripotent and erythroid progenitor cells and B and T lymphocyte precursors, as well as on mast cells. CD117 belongs to the tyrosine kinase receptor family and possesses five immunoglobulin-like C2-type domains and a cytoplasmic protein kinase domain. Binding of c-Kit ligand to CD117 induces dimerization and autophosphorylation, which activates several intracellular signaling pathways critical for the proliferation and differentiation of hematopoietic stem cells. Signaling by CD117 is modulated by phosphatases and by rapid endocytosis and degradation of the receptor. Mutations in CD117 are associated with various types of tumors and the piebald trait, an autosomal dominant abnormality of pigmentation. It has been reported that binding of the ACK2 antibody blocks the function of CD117.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD117 Antibody, Clone ACK2	60034, 60034.1	All	English
Product Information Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488	60034AD	All	English
Product Information Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, APC	60034AZ, 60034AZ.1	All	English
Product Information Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, FITC	60034FI	All	English
Product Information Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, PE	60034PE, 60034PE.1	All	English
Safety Data Sheet	Anti-Mouse CD117 Antibody, Clone ACK2	60034	All	English
Safety Data Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488	60034AD	All	English
Safety Data Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, APC	60034AZ, 60034AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, FITC	60034FI	All	English
Safety Data Sheet	Anti-Mouse CD117 Antibody, Clone ACK2, PE	60034PE, 60034PE.1	All	English

数据及文献

Data

Figure 1. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure 2. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 3. Data for AlexaFluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.