Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14_产品中心

抗体

Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14

Rat monoclonal IgG2a antibody against mouse CD62L (L-selectin)

概要

技术资料

数据及文献

概要

The MEL-14 antibody reacts with murine CD62L (L-Selectin or LECAM-1), an ~74 - 95 kDa type I glycoprotein expressed on the surface of a majority of leukocytes. CD62L mediates leukocyte-endothelial cell interactions through its association with ligands such as CD34, glyCAM-1, MAdCAM-1 and PSGL-1. It functions to facilitate lymphocyte rolling on the vascular endothelium, trafficking of lymphocytes to the lymph nodes, and homing of lymphocytes and neutrophils to sites of inflammation. Murine CD62L is expressed on most thymocytes, the highest expression levels being found on subsets of immunocompetent or dividing progenitor cells, as well as on neutrophils, eosinophils, monocytes, and subsets of B, T and NK cells. The MEL-14 antibody may be used together with antibodies to other cell surface markers to distinguish naïve, memory and effector T cells. The MEL-14 antibody has been shown to block migration of lymphocytes to lymph nodes and to inhibit leukocyte rolling.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14	60109, 60109.1	All	English
Product Information Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488	60109AD, 60109AD.1	All	English
Product Information Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC	60109AZ, 60109AZ.1	All	English
Product Information Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin	60109BT, 60109BT.1	All	English
Product Information Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC	60109FI, 60109FI.1, 60109FI.2	All	English
Product Information Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE	60109PE, 60109PE.1	All	English
Product Information Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5	60109PS, 60109PS.1	All	English
Safety Data Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14	60109, 60109.1	All	English
Safety Data Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488	60109AD, 60109AD.1	All	English
Safety Data Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC	60109AZ, 60109AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin	60109BT, 60109BT.1	All	English
Safety Data Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC	60109FI, 60109FI.1, 60109FI.2	All	English
Safety Data Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE	60109PE, 60109PE.1	All	English
Safety Data Sheet	Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5	60109PS, 60109PS.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram). (B) Flow cytometry analysis of mouse splenocytes (gated on CD4+ cells) processed with EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit and labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE and an anti-mouse CD44 antibody, FITC. Labeling of the Start splenocytes prior to cell isolation is shown (C).

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).