Anti-Mouse CD49b Antibody, Clone DX5_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD49b Antibody, Clone DX5

Rat monoclonal IgM antibody against mouse CD49b (integrin α2)

概要

技术资料

数据及文献

概要

The DX5 antibody reacts with murine CD49b (integrin α2), an ~150 kDa type 1 transmembrane glycoprotein that associates non-covalently with CD29 (integrin β1) to form the heterodimeric CD49b/CD29 complex known as VLA-2, a receptor for extracellular matrix proteins such as collagen, E-cadherin, fibronectin, and laminin. CD49b is highly expressed by platelets and is found on a majority of NK cells, on NKT cells, and on a small subset of CD8+ T cells; the latter population increases substantially following viral infection. CD49b is also expressed by several tissues, including intestine, kidney, mammary gland, and lung. The DX5 antibody is particularly useful for identifying NK cells in mice lacking the NK1.1 antigen. Binding of the DX5 antibody has not been observed to affect the function of the VLA-2 integrin. DX5 binding is, however, blocked by the clone HMα2 antibody.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ Mouse NK Cell Enrichment Kit (Catalog #19755).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD49b Antibody, Clone DX5	60020	All	English
Product Information Sheet	Anti-Mouse CD49b Antibody, Clone DX5, Alexa Fluor® 488	60020AD	All	English
Product Information Sheet	Anti-Mouse CD49b Antibody, Clone DX5, APC	60020AZ, 60020AZ.1	All	English
Product Information Sheet	Anti-Mouse CD49b Antibody, Clone DX5, Biotin	60020BT, 60020BT.1	All	English
Product Information Sheet	Anti-Mouse CD49b Antibody, Clone DX5, FITC	60020FI, 60020FI.1	All	English
Product Information Sheet	Anti-Mouse CD49b Antibody, Clone DX5, PE	60020PE, 60020PE.1	All	English
Product Information Sheet	Anti-Mouse CD49b Antibody, Clone DX5, Pacific Blue™	60020PB, 60020PB.1	All	English
Safety Data Sheet	Anti-Mouse CD49b Antibody, Clone DX5	60020	All	English
Safety Data Sheet	Anti-Mouse CD49b Antibody, Clone DX5, Alexa Fluor® 488	60020AD	All	English
Safety Data Sheet	Anti-Mouse CD49b Antibody, Clone DX5, APC	60020AZ, 60020AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD49b Antibody, Clone DX5, Biotin	60020BT, 60020BT.1	All	English
Safety Data Sheet	Anti-Mouse CD49b Antibody, Clone DX5, FITC	60020FI, 60020FI.1	All	English
Safety Data Sheet	Anti-Mouse CD49b Antibody, Clone DX5, PE	60020PE, 60020PE.1	All	English
Safety Data Sheet	Anti-Mouse CD49b Antibody, Clone DX5, Pacific Blue™	60020PB, 60020PB.1	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Alexa Fluor® 488 (Catalog #60074AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, PE (Catalog #60074PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118 (Catalog #60074), followed by a mouse anti-rat IgM antibody, FITC and Anti-Mouse CD45 antibody, Clone 30-F11, PE.

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, APC (Catalog #60074AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, Biotin (Catalog #60074BT), followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgM, kappa Isotype Control Antibody, Clone RTK2118, FITC (Catalog #60074FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 7. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD49b Antibody, Clone DX5, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgM, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.