Anti-Mouse CD45R (B220) Antibody, Clone RA3-6B2_产品中心

抗体

Anti-Mouse CD45R (B220) Antibody, Clone RA3-6B2

Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220)

概要

技术资料

数据及文献

概要

The RA3-6B2 antibody reacts with an epitope on the extracellular domain of the exon A‐restricted isoform of mouse CD45. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains (one of which is enzymatically active) that are involved in modulating the function of Src kinases such as Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 200 - 220 kDa. The exon A‐restricted isoform of mouse CD45, termed CD45R, contains the RA, RB, and RC regions and is the largest of the isoforms. CD45R is expressed by the B cell lineage from early pro‐B to mature B cells, with its expression downregulated during differentiation to plasma cells. CD45R is also expressed on subsets of T, NK, dendritic, and lymphokine-activated killer (LAK) cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2	60019, 100-0422	All	English
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488	60019AD, 60019AD.1	All	English
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC	60019AZ, 60019AZ.1	All	English
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin	60019BT, 100-0424, 100-0425	All	English
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC	60019FI, 100-0423	All	English
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5	60019PS, 60019PS.1	All	English
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™	60019PB, 60019PB.1	All	English
Product Information Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE	100-0420, 100-0421	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2	60019, 100-0422	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488	60019AD, 60019AD.1	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC	60019AZ, 60019AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin	60019BT, 100-0424, 100-0425	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC	60019FI, 100-0423	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5	60019PS, 60019PS.1	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™	60019PB, 60019PB.1	All	English
Safety Data Sheet	Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE	100-0420, 100-0421	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram).

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis fo C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 6. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Figure 7. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Figure 8. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).