Anti-Mouse CD25 Antibody, Clone PC61.5_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD25 Antibody, Clone PC61.5

Rat monoclonal IgG1 antibody against mouse CD25

概要

技术资料

数据及文献

概要

The PC61.5 antibody reacts with mouse CD25 (low-affinity interleukin‐2 receptor α chain or IL-2Rα), which is an ~55 kDa type 1 transmembrane glycoprotein expressed on T and B cell progenitors, activated (but not resting) T and B cells, regulatory T (Treg) cells, dendritic cells, and activated monocytes and macrophages. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. CD25 per se has low affinity for its IL-2 ligand but associates with CD122 (IL-2Rβ) and CD132 (IL-2Rγ) to form the high-affinity IL-2R receptor. CD25 is necessary for receptor clustering and induction of signaling pathways involved in the activation and proliferation of lymphocytes. Binding of the PC61.5 antibody inhibits binding of IL-2 to the low- and high-affinity receptors, which may result in inhibition of IL-2-dependent proliferation. The epitope recognized by PC61.5 is distinct from the IL-2 binding site and from the epitopes recognized by antibodies 7D4 and 3C7.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5	60009	All	English
Product Information Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, APC	60009AZ, 60009AZ.1	All	English
Product Information Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin	60009BT, 60009BT.1, 60009BT.2	All	English
Product Information Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, PE	60009PE, 60009PE.1	All	English
Product Information Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5	60009PS, 60009PS.1	All	English
Safety Data Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5	60009	All	English
Safety Data Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, APC	60009AZ, 60009AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin	60009BT, 60009BT.1, 60009BT.2	All	English
Safety Data Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, PE	60009PE, 60009PE.1	All	English
Safety Data Sheet	Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5	60009PS, 60009PS.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071 (Catalog #60075), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled withRat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC (Catalog #60075AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE (Catalog #60075PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 4. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a rat IgG1 isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a biotinylated rat IgG1 isotype control antibody followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.