Anti-Mouse CD24 Antibody, Clone M1/69_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD24 Antibody, Clone M1/69

Rat monoclonal IgG2b antibody against mouse CD24

概要

技术资料

数据及文献

概要

The M1/69 antibody reacts with murine CD24, an ~35 - 45 kDa glycoprotein expressed on lymphocytes, granulocytes, thymocytes, erythrocytes, epithelial cells, neurons, and dendritic cells. CD24 is differentially expressed during T and B cell development, and is not found on the majority of mature peripheral T cells or plasma cells. It is expressed on pro-B, pre-B and mature B cells, but expression is strongly down-regulated after B cell activation. CD24 is involved in regulating B cell apoptosis and in preventing terminal differentiation of B cells into antibody-secreting plasmablasts and plasma cells. It acts as an adhesion or co-stimulatory molecule and is involved in lymphocyte activation, proliferation and differentiation through homophilic interactions or by binding to ligands such as P-selectin (CD62P).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD24 Antibody, Clone M1/69	60099, 60099.1	All	English
Product Information Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488	60099AD, 60099AD.1	All	English
Product Information Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, APC	60099AZ, 60099AZ.1	All	English
Product Information Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, Biotin	60099BT, 60099BT.1	All	English
Product Information Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, FITC	60099FI, 60099FI.1	All	English
Product Information Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, PE	60099PE, 60099PE.1	All	English
Product Information Sheet 1	Anti-Mouse CD24 Antibody, Clone M1/69, PE	60099PE.1	All	English
Product Information Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5	60099PS, 60099PS.1	All	English
Safety Data Sheet	Anti-Mouse CD24 Antibody, Clone M1/69	60099, 60099.1	All	English
Safety Data Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488	60099AD, 60099AD.1	All	English
Safety Data Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, APC	60099AZ, 60099AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, Biotin	60099BT, 60099BT.1	All	English
Safety Data Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, FITC	60099FI, 60099FI.1	All	English
Safety Data Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, PE	60099PE, 60099PE.1	All	English
Safety Data Sheet	Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5	60099PS, 60099PS.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, Alexa Fluor® 488 (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) (solid line histogram).

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 6. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Figure 7. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Publications (1)

Stem cells (Dayton, Ohio) 2013 Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function Pond AC et al.

Abstract

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Abstract

Fibroblast growth factor (FGF) signaling plays an important role in embryonic stem cells and adult tissue homeostasis, but the function of FGFs in mammary gland stem cells is less well defined. Both FGFR1 and FGFR2 are expressed in basal and luminal mammary epithelial cells (MECs), suggesting that together they might play a role in mammary gland development and stem cell dynamics. Previous studies have demonstrated that the deletion of FGFR2 resulted only in transient developmental defects in branching morphogenesis. Using a conditional deletion strategy, we investigated the consequences of FGFR1 deletion alone and then the simultaneous deletion of both FGFR1 and FGFR2 in the mammary epithelium. FGFR1 deletion using a keratin 14 promoter-driven Cre-recombinase resulted in an early, yet transient delay in development. However, no reduction in functional outgrowth potential was observed following limiting dilution transplantation analysis. In contrast, a significant reduction in outgrowth potential was observed upon the deletion of both FGFR1 and FGFR2 in MECs using adenovirus-Cre. Additionally, using a fluorescent reporter mouse model to monitor Cre-mediated recombination, we observed a competitive disadvantage following transplantation of both FGFR1/R2-null MECs, most prominently in the basal epithelial cells. This correlated with the complete loss of the mammary stem cell repopulating population in the FGFR1/R2-attenuated epithelium. FGFR1/R2-null MECs were partially rescued in chimeric outgrowths containing wild-type MECs, suggesting the potential importance of paracrine mechanisms involved in the maintenance of the basal epithelial stem cells. These studies document the requirement for functional FGFR signaling in mammary stem cells during development.

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