Anti-Mouse CD19 Antibody, Clone 6D5_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD19 Antibody, Clone 6D5

Rat monoclonal IgG2a antibody against mouse CD19

概要

技术资料

数据及文献

概要

The 6D5 antibody reacts with CD19, an ~95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells. Expression is down-regulated but persists in terminally differentiated plasma cells. CD19 is also found on follicular dendritic cells. By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, which promotes binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes. Clone 6D5 recognizes the same epitope as clone ID3 in cross-competition assays.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD19 Antibody, Clone 6D5	60006	All	English
Product Information Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488	60006AD, 60006AD.1	All	English
Product Information Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, APC	60006AZ, 60006AZ.1	All	English
Product Information Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, Biotin	60006BT, 60006BT.1	All	English
Product Information Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, FITC	60006FI, 60006FI.1	All	English
Product Information Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, PE	60006PE, 60006PE.1	All	English
Product Information Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™	60006PB, 60006PB.1	All	English
Safety Data Sheet	Anti-Mouse CD19 Antibody, Clone 6D5	60006	All	English
Safety Data Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488	60006AD, 60006AD.1	All	English
Safety Data Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, APC	60006AZ, 60006AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, Biotin	60006BT, 60006BT.1	All	English
Safety Data Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, FITC	60006FI, 60006FI.1	All	English
Safety Data Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, PE	60006PE, 60006PE.1	All	English
Safety Data Sheet	Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™	60006PB, 60006PB.1	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (open histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (open histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076) followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, APC (filled histogram) or a rat IgG2a, kappa APC isotype control antibody (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2a, kappa biotin isotype control antibody followed by SAV APC (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 6D5, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).