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概要
The 2.4G2 antibody reacts with a shared epitope of Fc-gamma receptors CD16 (FcRIII) and CD32 (FcRII) in the mouse. CD16 and CD32 are low affinity receptors for the Fc portion of IgG, with CD32 having a higher affinity for IgG aggregates and immune complexes. CD16 and CD32 are expressed on B cells, monocytes, macrophages, NK cells, neutrophils, granulocytes, mast cells, and dendritic cells. The binding of immunoglobulins to Fc receptors is involved in multiple immunological functions, including phagocytosis, cytotoxocity, and the modulation of immune responses. The 2.4G2 antibody blocks the interaction between IgG and CD16 or CD32, and therefore is used as a pre-treatment to minimize non-specific binding of antibodies to FcR-bearing cells in applications such as flow cytometry, cell sorting, and immunofluorescence.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2 | 60161, 60161.1, 60161.2 | All | English |
| Product Information Sheet | Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, FITC | 60161FI, 60161FI.1 | All | English |
| Safety Data Sheet | Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2 | 60161, 60161.1, 60161.2 | All | English |
| Safety Data Sheet | Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, FITC | 60161FI, 60161FI.1 | All | English |
数据及文献
Data
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD16/CD32 Antibody, Clone 2.4G2, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI; solid line histogram).
