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概要
The DX1 antibody reacts with dextran, a natural polysaccharide consisting of linear chains of alpha-1,6-linked D-glucopyranose residues with short side chains (mostly 1 - 2 glucose units) 1,3- or 1,4-linked to the backbone of the biopolymer. Dextran has several uses as an additive in food, in lacquers, as a plasma volume expander, and as a coating for particles used in bioimaging or cell separation applications. The DX1 antibody was raised against isomaltotetraose (four sugar units in length) but binds better to longer polymer chains. It is also known to bind to amino-dextran, though with lower affinity. This antibody can be used to detect cells labelled with dextran-coated nanoparticles during StemSep™ and EasySep™ cell separation procedures or for other applications that require the detection of dextran.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051) and EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052).
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051) and EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet |
产品名称
Anti-Dextran Antibody, Clone DX1
|
60026 | All | 语言 English |
| Product Information Sheet |
产品名称
Anti-Dextran Antibody, Clone DX1, FITC
|
60026FI, 60026FI.1 | All | 语言 English |
| Safety Data Sheet |
产品名称
Anti-Dextran Antibody, Clone DX1
|
60026 | 全部 | 语言 English |
| Safety Data Sheet |
产品名称
Anti-Dextran Antibody, Clone DX1, FITC
|
60026FI, 60026FI.1 | 全部 | 语言 English |
数据及文献
数据
Figure 1. Data for Unconjugated
(A) Competitive ELISA showing effect of free dextran (filled circles) or sucrose (empty circles) on the binding of Anti-Dextran Antibody, Clone DX1 to immobilized dextran. (B) Western blot analysis of a dextran-conjugated carrier protein with Anti-Dextran Antibody, Clone DX1. Lane 1, unconjugated carrier protein (negative control); lane 2, carrier protein after conjugation to dextran.
Figure 2. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770) and labeled with Anti-Dextran, Clone DX1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (open histogram).
