Anti-Mouse CD8a Antibody, Clone 53-6.7_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD8a Antibody, Clone 53-6.7

Rat monoclonal IgG2a antibody against mouse, toad CD8a

概要

技术资料

数据及文献

概要

The 53-6.7 antibody reacts with murine CD8a, a 32 - 34 kDa type I transmembrane glycoprotein which is a subunit of CD8. CD8 is a disulfide-bonded dimer, found either as a heterodimer of CD8a (α) and CD8b (β) subunits (i.e., αβ) or a homodimer (αα). CD8 acts as a co-receptor to the T cell receptor (TCR) during T cell activation by binding MHC Class I molecules presented by an antigen-presenting cell. It functions to strengthen the association between the TCR and MHC I-antigen complex and to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. The CD8a chain binds to the alpha-3 domain of class I MHC molecules. CD8 is expressed in the αβ form by a majority of thymocytes and a subset of mature peripheral blood T cells (T cytotoxic cells), and in the ββ form by γδ T cells, a subset of intestinal intraepithelial lymphocytes and dendritic cells. The 53-6.7 antibody reportedly blocks antigen presentation via MHC class I and inhibits IL-2-dependent T cell responses.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD8+ T Cell Isolation Kit (Catalog #19853) and EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7	60023	All	English
Product Information Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488	60023AD, 60023AD.1	All	English
Product Information Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, APC	60023AZ, 60023AZ.1	All	English
Product Information Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin	60023BT, 60023BT.1	All	English
Product Information Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC	60023FI, 60023FI.1	All	English
Product Information Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, PE	60023PE, 60023PE.1	All	English
Product Information Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5	60023PS, 60023PS.1	All	English
Safety Data Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7	60023	All	English
Safety Data Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488	60023AD, 60023AD.1	All	English
Safety Data Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, APC	60023AZ, 60023AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin	60023BT, 60023BT.1	All	English
Safety Data Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC	60023FI, 60023FI.1	All	English
Safety Data Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, PE	60023PE, 60023PE.1	All	English
Safety Data Sheet	Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5	60023PS, 60023PS.1	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).