Anti-Mouse CD4 Antibody, Clone RM4-5_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD4 Antibody, Clone RM4-5

Rat monoclonal IgG2a antibody against mouse CD4

概要

技术资料

数据及文献

概要

The RM4-5 antibody reacts with mouse CD4, an ~55 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobulin (Ig) superfamily; CD4 contains four extracellular Ig-like domains. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T helper/inducer cells), and at lower levels on dendritic cells. In the mouse, CD4 is not expressed by monocytes/macrophages. CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Binding of the RM4-5 antibody to CD4 inhibits ligand binding in vitro; binding of the RM4-5 antibody can be blocked by the clone GK1.5 antibody.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852) and EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit (Catalog #18782).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5	60017	All	English
Product Information Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488	60017AD, 60017AD.1	All	English
Product Information Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, APC	60017AZ, 60017AZ.1	All	English
Product Information Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin	60017BT, 60017BT.1	All	English
Product Information Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, FITC	60017FI, 60017FI.1	All	English
Product Information Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, PE	60017PE, 60017PE.1	All	English
Product Information Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5	60017PS, 60017PS.1	All	English
Safety Data Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5	60017	All	English
Safety Data Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488	60017AD, 60017AD.1	All	English
Safety Data Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, APC	60017AZ, 60017AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin	60017BT, 60017BT.1	All	English
Safety Data Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, FITC	60017FI, 60017FI.1	All	English
Safety Data Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, PE	60017PE, 60017PE.1	All	English
Safety Data Sheet	Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5	60017PS, 60017PS.1	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD; solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE; solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ; solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin followed by streptavidin (SAV) APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT) followed by SAV APC (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI; solid line histogram).

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).