Anti-Mouse CD3e Antibody, Clone 145-2C11_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Mouse CD3e Antibody, Clone 145-2C11

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e

概要

技术资料

数据及文献

概要

The 145-2C11 antibody reacts with the ~20 kDa CD3ε subunit of the mouse T cell receptor (TCR)/CD3 complex, which is expressed on the surface of circulating mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severed combined immunodeficiency (SCID). The 145-2C11 antibody has been used for in vitro functional (blocking and activation) assays and has been reported to block binding by the clone 17A2 antibody. The 145-2C11 antibody is not recommended for use with formalin-fixed, paraffin-embedded sections.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse T Cell Isolation Kit (Catalog #19851) and EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11	60015	All	English
Product Information Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488	60015AD	All	English
Product Information Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, APC	60015AZ, 60015AZ.1	All	English
Product Information Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin	60015BT, 60015BT.1	All	English
Product Information Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC	60015FI, 60015FI.1	All	English
Product Information Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, PE	60015PE, 60015PE.1	All	English
Product Information Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5	60015PS, 60015PS.1	All	English
Safety Data Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11	60015	All	English
Safety Data Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488	60015AD	All	English
Safety Data Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, APC	60015AZ, 60015AZ.1	All	English
Safety Data Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin	60015BT, 60015BT.1	All	English
Safety Data Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC	60015FI, 60015FI.1	All	English
Safety Data Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, PE	60015PE, 60015PE.1	All	English
Safety Data Sheet	Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5	60015PS, 60015PS.1	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488 (filled histogram) or an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (filled histogram) or an Armenian hamster IgG PE isotype control antibody (solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, followed by an anti-hamster IgG antibody, FITC (filled histogram), or a hamster (Armenian) IgG isotype control antibody followed by an anti-hamster IgG antibody, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (filled histogram) or an Armenian hamster IgG APC isotype control antibody (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin followed by streptavidin (SAV) APC (filled histogram) or an Armenian hamster IgG biotin isotype control antibody followed by SAV APC (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC (filled histogram) or an Armenian hamster IgG FITC isotype control antibody (solid line histogram).

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5 (filled histogram) or an Armenian hamster IgG isotype control antibody, PerCP-Cy5.5 (solid line histogram).