Anti-Human p63 (deltaN) Antibody, Clone Poly6190_产品中心

抗体

Anti-Human p63 (deltaN) Antibody, Clone Poly6190

Rabbit polyclonal antibody against human p63 (deltaN)

概要

技术资料

数据及文献

概要

The Poly6190 antibody reacts with the deltaN isoforms of human p63, a member of the p53 family of transcription factors. Alternative splicing of the p63 transcript gives rise to two classes of isoforms. The TA isoforms (TA p63-α, -β, -γ) possess an N-terminal transactivation domain, while the deltaN isoforms (deltaN p63-α, -β, -γ) lack the transactivation domain. The TA isoforms are capable of transactivating p53 targets, whereas they antagonize p53. The deltaN isoforms are generally dominant to the TA isoforms and repress the latter. The p63 protein is localized to the nucleus and plays an important role in epithelial stem cell regeneration, epidermal morphogenesis, limb development, and stratification.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human p63 (deltaN) Antibody, Clone Poly6190	60154	All	English
Safety Data Sheet	Anti-Human p63 (deltaN) Antibody, Clone Poly6190	60154	All	English

数据及文献

Data

(A) Primary human airway epithelial cells were expanded in a submerged culture with PneumaCult™-Ex Medium (Catalog #05008) and labeled with Anti- Human p63 (deltaN) Antibody, Clone Poly6190, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594. (B) DAPI counterstaining of the cells shown in figure (A). (C) Primary human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface were cryo-sectioned and labeled with Anti-Human p63 (deltaN) Antibody, Clone Poly6190 (red), followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and antihuman NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (D) Western blot analysis of non-transfected HEK293 cell lysates (negative control; lane 1) and p63 (deltaN)-transfected HEK293 cell lysates (lane 2). Lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with Anti-Human p63 (deltaN) Antibody, Clone Poly6190. Proteins were visualized using an HRP-conjugated donkey anti-rabbit IgG secondary antibody and chemiluminescence detection.