Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2_产品中心

概要

The HIR2 (GA-R2) antibody recognizes an epitope common to the N-terminal region of human CD235a (glycophorin A) and CD235b (glycophorin B), homologous type I sialoglycoproteins of the erythrocyte (red blood cell) membrane that bear the antigenic determinants for the MN and Ss blood groups. CD235ab is expressed on early- and late-stage erythroblasts, mature erythrocytes, and erythroid cell lines such as K562 and HEL, but not on other cell types. CD235a is abundantly expressed whereas CD235b is a relatively minor membrane component. The proteins are believed to provide a large mucin-like surface to erythrocytes that acts to minimize aggregation in the circulation. The HIR2 antibody binds with higher affinity to CD235a than CD235b, and agglutinates untreated erythrocytes.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2	60111, 60111.1	All	English
Product Information Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, APC	60111AZ, 60111AZ.1	All	English
Product Information Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, Biotin	60111BT, 60111BT.1	All	English
Product Information Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, FITC	60111FI, 60111FI.1	All	English
Product Information Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, PE	60111PE, 60111PE.1	All	English
Safety Data Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2	60111, 60111.1	All	English
Safety Data Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, APC	60111AZ, 60111AZ.1	All	English
Safety Data Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, Biotin	60111BT, 60111BT.1	All	English
Safety Data Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, FITC	60111FI, 60111FI.1	All	English
Safety Data Sheet	Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, PE	60111PE, 60111PE.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, APC (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (Catalog #60072AZ, solid line histogram).

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, Biotin followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin (Catalog #60072BT) followed by SAV APC (solid line histogram).

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, FITC (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (Catalog #60072FI, solid line histogram).

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human whole blood labeled with Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2, PE (filled histogram) or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE, solid line histogram).