Anti-Human CD184 (CXCR4) Antibody, Clone 12G5_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Human CD184 (CXCR4) Antibody, Clone 12G5

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4)

概要

技术资料

数据及文献

概要

The 12G5 antibody reacts with CD184 (CXCR4 or fusin), an ~45 kDa seven transmembrane G-protein-coupled receptor expressed on the surface of a broad range of cells, including T and B cells, dendritic cells, monocytes, macrophages, granulocytes, platelets, endothelial and epithelial cells, astrocytes and neurons. At least two isoforms of CD184 are generated by alternate mRNA splicing. Ligands for CD184 include the CXC chemokine SDF-1 and ubiquitin. CD184 has functional roles in mediating chemotaxis, B cell lymphopoiesis and myelopoiesis, cardiogenesis, vascularization and neuronal guidance. CD184 acts as a co-receptor for infection by some T cell-tropic isolates of HIV-1 and as an alternative receptor for CD4-independent infection by HIV-2; functions that are blocked by binding of the 12G5 antibody. The antibody also partially inhibits SDF-1-induced chemotaxis and calcium influx. In differentiation studies, CD184 is used along with other markers such as CD117 to monitor the emergence of definitive endoderm.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5	60089	All	English
Product Information Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC	60089AZ, 60089AZ.1	All	English
Product Information Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin	60089BT	All	English
Product Information Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE	60089PE, 60089PE.1	All	English
Safety Data Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5	60089	All	English
Safety Data Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC	60089AZ, 60089AZ.1	All	English
Safety Data Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin	60089BT	All	English
Safety Data Sheet	Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE	60089PE, 60089PE.1	All	English

数据及文献

Data

Figure 1. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram).

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram).

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram). (B) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (Catalog #60087AZ) and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE. The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184. (C) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE.

Figure 4. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, followed by anti-mouse IgG, FITC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by anti-mouse IgG, FITC (solid line histogram).