Anti-Human CD83 Antibody, Clone HB15e_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Human CD83 Antibody, Clone HB15e

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83

概要

技术资料

数据及文献

概要

The HB15e antibody reacts with CD83, an ~45 kDa type I transmembrane glycoprotein and member of the Siglec protein family, containing an extracellular V-type Ig-like domain and a C-terminal cytoplasmic tail. A soluble form of CD83 has also been identified. CD83 is expressed predominantly on circulating and tissue dendritic cells, including Langerhan cells and interdigitating reticulum cells. It is expressed weakly on activated lymphocytes and may also be found on neutrophils, monocytes and macrophages. Expression of CD83 is typically transient, except on dendritic cells, and correlates with upregulation of HLA class II antigen. Its function, though ill-defined, is essential for the development and maintenance of the immune system and evidence suggests it may be involved in cell adhesion and signaling, antigen presentation, and lymphocyte activation. The soluble form of CD83 appears to be immunosuppressive. The epitope on CD83 bound by the HB15e antibody reportedly overlaps with that of antibody 1E11.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD83 Antibody, Clone HB15e	60107, 60107.1	All	English
Product Information Sheet	Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488	60107AD	All	English
Product Information Sheet	Anti-Human CD83 Antibody, Clone HB15e, APC	60107AZ, 60107AZ.1	All	English
Product Information Sheet	Anti-Human CD83 Antibody, Clone HB15e, Biotin	60107BT, 60107BT.1	All	English
Product Information Sheet	Anti-Human CD83 Antibody, Clone HB15e, FITC	60107FI, 60107FI.1	All	English
Product Information Sheet	Anti-Human CD83 Antibody, Clone HB15e, PE	60107PE, 60107PE.1, 60107PE.2	All	English
Safety Data Sheet	Anti-Human CD83 Antibody, Clone HB15e	60107, 60107.1	All	English
Safety Data Sheet	Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488	60107AD	All	English
Safety Data Sheet	Anti-Human CD83 Antibody, Clone HB15e, APC	60107AZ, 60107AZ.1	All	English
Safety Data Sheet	Anti-Human CD83 Antibody, Clone HB15e, Biotin	60107BT, 60107BT.1	All	English
Safety Data Sheet	Anti-Human CD83 Antibody, Clone HB15e, FITC	60107FI, 60107FI.1	All	English
Safety Data Sheet	Anti-Human CD83 Antibody, Clone HB15e, PE	60107PE, 60107PE.1, 60107PE.2	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 (solid line histogram).

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).