Anti-Human CD45RO Antibody, Clone UCHL1_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Human CD45RO Antibody, Clone UCHL1

Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO

概要

技术资料

数据及文献

概要

The UCHL1 antibody reacts with an extracellular epitope on CD45RO, which is the shortest isoform of CD45, a type I transmembrane glycoprotein and member of the protein tyrosine phosphatase family (receptor class 1/6 subfamily). Alternative splicing of exons 4, 5 and 6 that encode the extracellular RA, RB and RC polypeptides of CD45 gives rise to up to 8 isoforms with molecular masses of 180 - 240 kDa. Excision of all three exons generates the ~180 kDa CD45RO isoform, which is expressed on activated and memory (but not naïve) T cells, some B cell subsets, activated monocytes and macrophages, and granulocytes. CD45RO enhances both T cell receptor- and B cell receptor-mediated activation and is a known ligand for CD22 on B cells. The UCHL1 antibody has been used to identify T cell lymphomas and leukemia and is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. The proportion of CD45RO+ (memory) T cells typically increases with age. The UCHL1 epitope is destroyed by treatment with neuraminidase or O-glycosidase.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD45RO Antibody, Clone UCHL1	60097	All	English
Product Information Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488	60097AD	All	English
Product Information Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, APC	60097AZ, 60097AZ.1	All	English
Product Information Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, Biotin	60097BT	All	English
Product Information Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, FITC	60097FI	All	English
Product Information Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, PE	60097PE, 60097PE.1	All	English
Product Information Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5	60097PS, 60097PS.1	All	English
Safety Data Sheet	Anti-Human CD45RO Antibody, Clone UCHL1	60097	All	English
Safety Data Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488	60097AD	All	English
Safety Data Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, APC	60097AZ, 60097AZ.1	All	English
Safety Data Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, Biotin	60097BT	All	English
Safety Data Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, FITC	60097FI	All	English
Safety Data Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, PE	60097PE, 60097PE.1	All	English
Safety Data Sheet	Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5	60097PS, 60097PS.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat anti-mouse IgG2a antibody, PE (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, followed by a rat antimouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on lymphocytes) and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, followed by a rat anti-mouse IgG2a antibody, PE and an anti-human CD45RA antibody, FITC.

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488 and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and an anti-human CD45RA antibody, APC.

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMSs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ) (solid line histogram) (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, APC and an anti-human CD45RA antibody, FITC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC and an anti-human CD45RA antibody, FITC.

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, Biotin, followed by streptavidin (SAV) APC and an anti-human CD45RA antibody, FITC (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC and an anti-human CD45RA antibody, FITC.

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, FITC and an anti-human CD45RA antibody, APC. (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (Catalog #60071FI) and an anti-human CD45RA antibody, APC.

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) (solid line histogram). (B) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PE and an anti-human CD45RA antibody, APC. (C) Flow cytometry analysis of human PBMCs (gated on CD3+ cells) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE and an anti-human CD45RA antibody, APC.

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).
(B) Flow cytometry analysis of human PBMCs labeled with a mouse IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and an anti-human CD45RA antibody, FITC. Cells were gated on CD3+ cells (T lymphocytes).