Anti-Human CD45 Antibody, Clone HI30_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Human CD45 Antibody, Clone HI30

Mouse monoclonal IgG1 antibody against human, chimpanzee CD45

概要

技术资料

数据及文献

概要

The HI30 antibody reacts with all isoforms of CD45, a type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets, and plasma cells; expression of CD45 is lost during differentiation of these cell types. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains (one of which is enzymatically active) that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, 6) encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 - 240 kDa. The leukocyte common antigen (LCA), the region recognized by the HI30 antibody, is an extracellular region located proximal to the membrane and common to all isoforms of CD45.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051), EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052) and EasySep™ Human CD45 Depletion Kit (Catalog #18259; partial blocking may be observed).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30	60018	All	English
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30, Alexa Fluor® 488	60018AD, 60018AD.1	All	English
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30, APC	60018AZ, 60018AZ.1, 100-0467	All	English
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30, Biotin	60018BT, 60018BT.1	All	English
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30, FITC	60018FI, 60018FI.1, 100-0466	All	English
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30, PE	60018PE, 60018PE.1	All	English
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5	60018PS, 60018PS.1	All	English
Product Information Sheet	Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™	60018PB, 60018PB.1, 60018PB.2	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30	60018	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30, Alexa Fluor® 488	60018AD, 60018AD.1	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30, APC	60018AZ, 60018AZ.1, 100-0467	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30, Biotin	60018BT, 60018BT.1	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30, FITC	60018FI, 60018FI.1, 100-0466	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30, PE	60018PE, 60018PE.1	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5	60018PS, 60018PS.1	All	English
Safety Data Sheet	Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™	60018PB, 60018PB.1, 60018PB.2	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram).

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 8. Data for PB-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™ (filled histogram) or a mouse IgG1, kappa Pacific Blue™ isotype control antibody (solid line histogram).