Anti-Human CD43 Antibody, Clone CD43-10G7_产品中心_北京砺达辰生物科技有限公司

概要

The CD43-10G7 antibody reacts with human CD43, a type I transmembrane sialoglycoprotein expressed on the surface of most human hematopoietic cells, including thymocytes, peripheral T cells, NK cells, monocytes, granulocytes, some B cells (activated B cells and an early B cell subset in bone marrow), plasma cells, and hematopoietic progenitor cells. Low expression levels are observed on platelets. CD43 exhibits different glycoforms in the range of ~95 - 135 kDa, depending on the cell type. It binds ligands such as CD54 and CD169 (Siglec-1) and plays regulatory roles in cell adhesion and activation, apoptosis and tumor progression, and is used a marker for discriminating normal from neoplastic T cells and B cells within tissues. Binding of the CD43-10G7 antibody to CD43 on neutrophils reportedly induces homotypic cell adhesion.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD43 Antibody, Clone CD43-10G7	60085	All	English
Product Information Sheet	Anti-Human CD43 Antibody, Clone CD43-10G7, APC	60085AZ, 60085AZ.1	All	English
Product Information Sheet	Anti-Human CD43 Antibody, Clone CD43-10G7, PE	60085PE, 60085PE.1	All	English
Safety Data Sheet	Anti-Human CD43 Antibody, Clone CD43-10G7	60085	All	English
Safety Data Sheet	Anti-Human CD43 Antibody, Clone CD43-10G7, APC	60085AZ, 60085AZ.1	All	English
Safety Data Sheet	Anti-Human CD43 Antibody, Clone CD43-10G7, PE	60085PE, 60085PE.1	All	English

数据及文献

Data

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, APC and Anti-Human CD45 Antibody, Clone HI30, PE (Catalog #60018PE).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, APC and Anti-Human CD45 Antibody, Clone HI30, PE. Note: CD43 is not expressed on resting B cells.
(C) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa APC isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, PE.

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, PE and Anti-Human CD45 Antibody, Clone HI30, APC. Note: CD43 is not expressed on resting B cells.
(C) Flow cytometry analysis of human buffy coat nucleated cells labeled with a mouse IgG1, kappa PE isotype control antibody and Anti-Human CD45 Antibody, Clone HI30, APC.

Figure 3. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD43 Antibody, Clone CD43-10G7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).