Anti-Human CD33 Antibody, Clone HIM3-4_产品中心_北京砺达辰生物科技有限公司

概要

The HIM3-4 antibody reacts with CD33 (Siglec-3), an ~67 - 75 kDa type 1 transmembrane sialoadhesion protein of the immunoglobulin superfamily. CD33 is expressed on committed myeloid progenitor cells, monocytes, granulocytes, dendritic cells, mast cells, resident macrophages, and subsets of B cells and activated T and NK cells. CD33 is a major marker used to differentiate myeloid from lymphoid or erythroid leukemias. In peripheral blood it is expressed at high levels on monocytes and at lower levels on granulocytes. CD33 functions as a sialic acid-dependent cell adhesion molecule and mediates protein-glycan and protein-protein interactions between cells. It also has a putative role as an inhibitory receptor during the immune response. HIM3-4 reportedly binds to the extracellular C2 Ig domain of CD33, and does not block subsequent binding of several other anti-CD33 clones, including P67.6, WM53, and WM54. Two isoforms designated CD33M and CD33m have been identified and among these clones only HIM3-4 recognizes both isoforms.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD33 Antibody, Clone HIM3-4	60096	All	English
Product Information Sheet	Anti-Human CD33 Antibody, Clone HIM3-4, FITC	60096FI, 60096FI.1	All	English
Product Information Sheet	Anti-Human CD33 Antibody, Clone HIM3-4, PE	60096PE, 60096PE.1	All	English
Safety Data Sheet	Anti-Human CD33 Antibody, Clone HIM3-4	60096	All	English
Safety Data Sheet	Anti-Human CD33 Antibody, Clone HIM3-4, FITC	60096FI, 60096FI.1	All	English
Safety Data Sheet	Anti-Human CD33 Antibody, Clone HIM3-4, PE	60096PE, 60096PE.1	All	English

数据及文献

Data

Figure 1. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD33 Antibody, Clone HIM3-4, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD33 Antibody, Clone HIM3-4, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse Ig1, kappa FITC isotype control antibody is shown (open histogram).

Figure 2. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD33 Antibody, Clone HIM3-4, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells labeled with Anti-Human CD33 Antibody, Clone HIM3-4, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE) (solid line histogram).