Anti-Human CD16 Antibody, Clone 3G8_产品中心_北京砺达辰生物科技有限公司

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Anti-Human CD16 Antibody, Clone 3G8

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD16

概要

技术资料

数据及文献

概要

The 3G8 antibody reacts with CD16 (FcγRIII or low affinity IgG receptor III), a type 1 transmembrane glycoprotein belonging to the Ig superfamily. CD16 exists in two distinct isoforms: CD16a, a 50 - 65 kDa form expressed on NK cells, activated monocytes, macrophages, and placental trophoblasts, and CD16b, an ~48 kDa glycosylphosphatidylinositol (GPI)-anchored form expressed on neutrophils, basophils and eosinophils and found as at least two polymorphic variants, termed NA1 and NA2. CD16 binds weakly to the Fc region of monomeric, aggregated or complexed IgG, particularly the IgG1 and IgG3 isotypes. Binding of IgG to either CD16 isoform induces signaling pathways that modulate several types of responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release and proliferation. CD16/IgG interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the 3G8 antibody may be employed as a blocking antibody to reduce non-specific binding.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD16 Antibody, Clone 3G8	60041, 60041.1	All	English
Product Information Sheet	Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor® 488	60041AD, 60041AD.1	All	English
Product Information Sheet	Anti-Human CD16 Antibody, Clone 3G8, APC	60041AZ, 60041AZ.1	All	English
Product Information Sheet	Anti-Human CD16 Antibody, Clone 3G8, Biotin	60041BT, 60041BT.1	All	English
Product Information Sheet	Anti-Human CD16 Antibody, Clone 3G8, FITC	60041FI, 60041FI.1	All	English
Product Information Sheet	Anti-Human CD16 Antibody, Clone 3G8, PE	60041PE, 60041PE.1	All	English
Safety Data Sheet	Anti-Human CD16 Antibody, Clone 3G8	60041, 60041.1	All	English
Safety Data Sheet	Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor® 488	60041AD, 60041AD.1	All	English
Safety Data Sheet	Anti-Human CD16 Antibody, Clone 3G8, APC	60041AZ, 60041AZ.1	All	English
Safety Data Sheet	Anti-Human CD16 Antibody, Clone 3G8, Biotin	60041BT, 60041BT.1	All	English
Safety Data Sheet	Anti-Human CD16 Antibody, Clone 3G8, FITC	60041FI, 60041FI.1	All	English
Safety Data Sheet	Anti-Human CD16 Antibody, Clone 3G8, PE	60041PE, 60041PE.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor® 488 (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD) (solid line histogram).

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ) (solid line histogram).

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC (solid line histogram).

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE) (solid line histogram).