Anti-Human CD11b Antibody, Clone ICRF44_产品中心_北京砺达辰生物科技有限公司

抗体

Anti-Human CD11b Antibody, Clone ICRF44

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD11b

概要

技术资料

数据及文献

概要

The ICRF44 antibody reacts with an extracellular epitope on CD11b (integrin αM), an ~170 kDa type 1 transmembrane glycoprotein which associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. Through its interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, heparin, and fibrinogen, Mac-1 influences several processes, including the adherence of neutrophils and monocytes to stimulated endothelium, and phagocytosis of complement-coated particles. CD11b is expressed on the surface of granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. CD11b is a relatively late marker for myeloid differentiation, and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. Certain mutations in CD11b give rise to the disorder systemic lupus erythematosus. The ICRF44 antibody reportedly inhibits leukocyte aggregation in response to the chemoattractant fMLP.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human Monocyte Enrichment Kit (Catalog #19059), and for labeling human mesenchymal cells grown in MesenCult™ Proliferation Kit (Human; Catalog #05411).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD11b Antibody, Clone ICRF44	60040	All	English
Product Information Sheet	Anti-Human CD11b Antibody, Clone ICRF44, Alexa Fluor® 488	60040AD, 60040AD.1	All	English
Product Information Sheet	Anti-Human CD11b Antibody, Clone ICRF44, APC	60040AZ, 60040AZ.1	All	English
Product Information Sheet	Anti-Human CD11b Antibody, Clone ICRF44, Biotin	60040BT, 60040BT.1	All	English
Product Information Sheet	Anti-Human CD11b Antibody, Clone ICRF44, FITC	60040FI, 60040FI.1	All	English
Product Information Sheet	Anti-Human CD11b Antibody, Clone ICRF44, PE	60040PE, 60040PE.1	All	English
Safety Data Sheet	Anti-Human CD11b Antibody, Clone ICRF44	60040	All	English
Safety Data Sheet	Anti-Human CD11b Antibody, Clone ICRF44, Alexa Fluor® 488	60040AD, 60040AD.1	All	English
Safety Data Sheet	Anti-Human CD11b Antibody, Clone ICRF44, APC	60040AZ, 60040AZ.1	All	English
Safety Data Sheet	Anti-Human CD11b Antibody, Clone ICRF44, Biotin	60040BT, 60040BT.1	All	English
Safety Data Sheet	Anti-Human CD11b Antibody, Clone ICRF44, FITC	60040FI, 60040FI.1	All	English
Safety Data Sheet	Anti-Human CD11b Antibody, Clone ICRF44, PE	60040PE, 60040PE.1	All	English

数据及文献

Data

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD11b Antibody, Clone ICRF44, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD11b Antibody, Clone ICRF44, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat AntiMouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of human whole blood nucleated cells labeled with Anti-Human CD11b Antibody, Clone ICRF44, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).