Anti-Human CD4, Clone RPA-T4_产品中心_北京砺达辰生物科技有限公司

概要

The RPA-T4 antibody reacts with CD4, an ~59 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobulin (Ig) superfamily; CD4 contains four extracellular Ig-like domains (D1 - D4). The epitope for RPA-T4 has been localized to the D1 domain of the protein, which has a structure resembling an Ig variable domain. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T helper cells), and at lower levels by peripheral blood monocytes and macrophages. CD4 binds to a nonpolymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the affinity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases, such as Lck. Moreover, CD4 is a receptor for the human immunodeficiency virus (HIV). Binding of the RPA-T4 antibody blocks HIV binding and mixed lymphocyte reaction (MLR), and inhibits the activation of CD4+ T cells in vitro by interfering with the association of CD4 and HLA-DR. It also partially blocks the binding of antibody clone SK3. The epitope of the RPA-T4 antibody is reportedly sensitive to formaldehyde fixation.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human CD4, Clone RPA-T4, APC	100-0298, 100-0299, 100-0300	All	English
Product Information Sheet	Anti-Human CD4, Clone RPA-T4, FITC	100-0301, 100-0302, 100-0303	All	English
Product Information Sheet	Anti-Human CD4, Clone RPA-T4, PE	100-0304, 100-0305, 100-0306	All	English
Product Information Sheet	Anti-Human CD4, Clone RPA-T4	100-0307	All	English
Safety Data Sheet	Anti-Human CD4, Clone RPA-T4, APC	100-0298, 100-0299, 100-0300	All	English
Safety Data Sheet	Anti-Human CD4, Clone RPA-T4, FITC	100-0301, 100-0302, 100-0303	All	English
Safety Data Sheet	Anti-Human CD4, Clone RPA-T4, PE	100-0304, 100-0305, 100-0306	All	English
Safety Data Sheet	Anti-Human CD4, Clone RPA-T4	100-0307	All	English

数据及文献

Data

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, APC or a mouse IgG1, kappa APC isotype control antibody.

Figure 1. Data for Anti-Human CD4 Antibody, Clone RPA-T4, APC

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, FITC or a mouse IgG1, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Human CD4 Antibody, Clone RPA-T4, FITC

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, PE or a mouse IgG1, kappa PE isotype control antibody.

Figure 3. Data for Anti-Human CD4 Antibody, Clone RPA-T4, PE

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, followed by a goat anti-mouse IgG1 antibody, PE or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG1 antibody, PE.

Figure 4. Data for Anti-Human CD4 Antibody, Clone RPA-T4, Unconjugated

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, followed by a goat anti-mouse IgG1 antibody, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG1 antibody, PE (solid line histogram).