Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3_产品中心

抗体

Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III

概要

技术资料

数据及文献

概要

The 2G10-TB3 antibody reacts with beta-tubulin III, an ~50 - 55 kDa structural protein that is a constituent of tubulin. Tubulin is the major component of microtubules within the cytoskeleton and is assembled from heterodimers of alpha- and beta-tubulin subunits. The beta III isoform of tubulin, also known as neuron-specific class III beta-tubulin, is expressed primarily in neurons and is widely used as a marker to distinguish neurons from other cell types. Beta-tubulin III contributes to microtubule formation in neuronal cell bodies and axons, a function involving GTP binding, and plays roles in axonal transport, neuronal cell proliferation, and differentiation. It is highly expressed in several types of cancer and is a predictive and prognostic marker for various tumors, for example, being found in neoplastic but not in normal glial cells. The 2G10-TB3 antibody is expected to recognize all mammalian homologs of beta-tubulin III and the epitope has reportedly been mapped to the extreme C-terminal amino acids, EAQGPK.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3	60092, 60092.1	All	English
Safety Data Sheet	Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3	60092, 60092.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

(A) E18 cortical rat neurons were cultured using NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Human Beta-Tubulin III Antibody, Clone 2G10-TB3, followed by donkey anti-mouse IgG, Alexa Fluor® 488. Nuclei were counterstained with DAPI. Inset shows cells labeled with a mouse IgG2a, kappa isotype control antibody followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counterstained with DAPI. (B) Western blot analysis of denatured/reduced cell lysates with Anti-Beta Tubulin III Antibody, Clone 2G10-TB3. Lane 1, adult rat brain cortical cells; lane 2 (negative control), mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse & Rat).