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Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530

Rat monoclonal IgG2b, kappa isotype control antibody

概要
技术资料
数据及文献

概要

The RTK4530 antibody (IgG2b, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).

技术资料

Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 60077, 60077.1 All English
Product Information Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 60077AD All English
Product Information Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC 60077AZ, 60077AZ.1 All English
Product Information Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin 60077BT, 60077BT.1 All English
Product Information Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC 60077FI, 60077FI.1, 60077FI.2, 60077FI.3 All English
Product Information Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE 60077PE, 60077PE.1, 60077PE.2, 60077PE.3 All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 60077, 60077.1 All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 60077AD All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC 60077AZ, 60077AZ.1 All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin 60077BT, 60077BT.1 All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC 60077FI, 60077FI.1 All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC 60077FI.2, 60077FI.3 All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE 60077PE, 60077PE.1 All English
Safety Data Sheet Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE 60077PE.2, 60077PE.3 All English

数据及文献

Data

Data for unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Catalog #60030) followed by a mouse anti-rat IgG2b antibody, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488, Catalog #60030AD).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, APC, Catalog #60030AZ).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin, Catalog #60030BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, FITC, Catalog #60030FI).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, PE, Catalog #60030PE).