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概要
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
技术资料
数据及文献
Data
Figure 1. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Catalog #60023) followed by a mouse anti-rat IgG2a antibody, FITC.
Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488, Catalog #60023AD).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, APC, Catalog #60023AZ).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, Catalog #60023BT) followed by SAV APC.
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC, Catalog #60023FI).
Figure 6. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (solid line histogram). Filled histogram shows labeling with a rat IgG2a, kappa positive control antibody (Anti-Mouse CD8a Antibody, Clone 53-6.7, PE, Catalog #60023PE).
