Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071_产品中心

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Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071

Rat monoclonal IgG1, kappa isotype control antibody

概要

技术资料

数据及文献

概要

The RTK2071 antibody (IgG1, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071	60075, 60075.1	All	English
Product Information Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488	60075AD	All	English
Product Information Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC	60075AZ, 60075AZ.1	All	English
Product Information Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin	60075BT, 60075BT.1	All	English
Product Information Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC	60075FI, 60075FI.1	All	English
Product Information Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE	60075PE, 60075PE.1	All	English
Safety Data Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071	60075, 60075.1	All	English
Safety Data Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488	60075AD	All	English
Safety Data Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC	60075AZ, 60075AZ.1	All	English
Safety Data Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin	60075BT, 60075BT.1	All	English
Safety Data Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC	60075FI, 60075FI.1	All	English
Safety Data Sheet	Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE	60075PE, 60075PE.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5 (Catalog #60009), followed by a mouse anti-rat IgG1 antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, Alexa Fluor® 488 and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, APC (Catalog #60009AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, Biotin (Catalog #60009BT) followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with an anti-mouse CD25 antibody, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Rat IgG1, kappa Isotype Control Antibody, Clone RTK2071, PE and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (Catalog #60015AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse CD25 Antibody, Clone PC61.5, PE (Catalog #60009PE) and Anti-Mouse CD3e Antibody, Clone 145-2C11, APC.