Mouse IgM, kappa Isotype Control Antibody, Clone MM-30_产品中心

概要

The MM-30 antibody (IgM, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specfic antibody and indicated non-specific binding that may result from binding to Fc receptors or other cell components. The MM-30 antibody reportedly recognizes keyhole limpet hemocyanin.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Mouse IgM, kappa Isotype Control Antibody, Clone MM30	60069	All	English
Product Information Sheet	Mouse IgM, kappa Isotype Control Antibody, Clone MM30, Alexa Fluor® 488	60069AD	All	English
Product Information Sheet	Mouse IgM, kappa Isotype Control Antibody, Clone MM30, PE	60069PE, 60069PE.1	All	English
Safety Data Sheet	Mouse IgM, kappa Isotype Control Antibody, Clone MM30	60069	All	English
Safety Data Sheet	Mouse IgM, kappa Isotype Control Antibody, Clone MM30, Alexa Fluor® 488	60069AD	All	English
Safety Data Sheet 1	Mouse IgM, kappa Isotype Control Antibody, Clone MM30, PE	60069PE, 60069PE.1	All	English
Safety Data Sheet 2	Mouse IgM, kappa Isotype Control Antibody, Clone MM30, PE	60069PE, 60069PE.1	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA160 Antibody, Clone TRA-1-60R; Catalog #60064) followed by goat anti-mouse IgG, FITC (filled histogram). (B) Human H1 ES cells were cultured in mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-81 Antibody, Clone TRA-1-81; Catalog #60065).

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488; Catalog #60064AD) (filled histogram).

Figure 3. Data for PE-Conjugated

Flow cytometry analysis of human H1 embryonic stem (ES) cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (solid line histogram), or a mouse IgM, kappa positive control antibody (Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE; Catalog #60064PE) (filled histogram).