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概要
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
技术资料
数据及文献
Data
Figure 1. Flow Cytometry Analysis of Human PBMCs
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC11, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7; Catalog #60008), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC. (B) Human induced pluripotent stem (iPS) cells were cultured in TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, followed by goat anti-mouse IgG, FITC (Inset), or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20 (Catalog #60093), followed by goat anti-mouse IgG, FITC (green).
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488, Catalog #60008AD). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488 (Catalog #60093AD, green).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, APC, Catalog #60008AZ).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Biotin, Catalog #60008BT) followed by SAV APC.
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, FITC, Catalog #60008FI).
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PE, Catalog #60008PE). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE (Catalog #60093PE).
Figure 7. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5, Catalog #60008PS).
