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Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173

Mouse monoclonal IgG2a, kappa isotype control antibody

概要
技术资料
数据及文献

概要

The MOPC-173 antibody (IgG2a, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).

数据及文献

Data

Flow Cytometry Analysis of Human PBMCs

Figure 1. Flow Cytometry Analysis of Human PBMCs

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Catalog #60097) followed by a rat anti-mouse IgG2a antibody, PE. (B) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit (Catalog #05712) on poly-lysine-coated glass coverslips, then fixed and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, followed by donkey anti-mouse IgG, Alexa Fluor® 488 and DAPI counterstaining (inset), or with a positive control antibody of the same isotype, Anti-Human Beta-Tubulin III Antibody, Clone AA10 (Catalog #60100), followed by donkey anti-mouse IgG, Alexa Fluor® 488 (green) and DAPI counterstaining (blue).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Alexa Fluor® 488, Catalog #60097AD). (B) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit (Catalog #05712) on poly-lysine-coated glass coverslips, then fixed and labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Inset) or with a positive control antibody of the same isotype, Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488 (Catalog #60100AD, green). DAPI counterstaining is shown in blue.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC- 173, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, APC, Catalog #60097AZ).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Biotin, Catalog #60097BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, FITC, Catalog #60097FI).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PE, Catalog #60097PE).