Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4_产品中心

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Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4

Mouse monoclonal IgG2a, kappa isotype control antibody

概要

技术资料

数据及文献

概要

The C1.18.4 antibody (IgG2a, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being employed. If a conjugated antibody is employed, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4	60108	All	English
Product Information Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC	60108AZ	All	English
Product Information Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC	60108FI	All	English
Product Information Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE	60108PE	All	English
Product Information Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5	60108PS	All	English
Safety Data Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4	60108	All	English
Safety Data Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC	60108AZ	All	English
Safety Data Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC	60108FI	All	English
Safety Data Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE	60108PE	All	English
Safety Data Sheet	Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5	60108PS	All	English

数据及文献

Data

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, followed by a rat anti-mouse IgG2a antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, Catalog #60097) followed by a rat anti-mouse IgG2a antibody, PE.

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, APC, Catalog #60097AZ).

Figure 3. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, FITC, Catalog #60097FI).

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PE, Catalog #60097PE).

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone C1.18.4, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2a, kappa positive control antibody (Anti-Human CD45RO Antibody, Clone UCHL1, PerCP-Cy5.5, Catalog #60097PS).