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概要
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
技术资料
数据及文献
Data
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Catalog #60107) followed by a rat anti-mouse IgG1 antibody, PE.(B) Human embryonic stem (ES) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by goat anti-mouse IgG, FITC (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11 (Catalog #60063), followed by goat anti-mouse IgG, FITC (green).
Figure 2. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488, Catalog #60107AD). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, Alexa Fluor® 488 (Catalog #60063AD, green).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, APC, Catalog #60107AZ).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Biotin, Catalog #60107BT) followed by SAV APC.
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, FITC, Catalog #60107FI).
Figure 6. Data for PE-Conjugated
(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, PE, Catalog #60107PE). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, PE (Catalog #60063PE, red).
Figure 7. Data for PS-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC- 21, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5; Catalog #60018PS).
