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概要
Ym1 is an ~45 kDa secreted protein synthesized by activated mouse peritoneal macrophages during the inflammatory response elicited by parasitic infections. Ym1 is a granule protein in neutrophils and has been purified from eosinophilic crystals that form in the bronchoalveolar lavage fluid of mev/mev and SHIP (SH2-containing inositol 5’ phosphatase) knockout mice. The eosinophilic crystals formed in mice are considered to be histologically identical to the Charcot-Leyden crystals found in humans. Ym1 is homologous to T lymphocyte-derived eosinophil chemotactic factor (ECF-L) but elicits only a weak chemotactic response in eosinophils. Ym1 binds to heparin, GlcN oligomers and heparan sulfate. It belongs to a protein family related to chitinases but does not possess chitinase activity.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Anti-Ym1 Antibody, Polyclonal | 60130 | All | English |
| Safety Data Sheet | Anti-Ym1 Antibody, Polyclonal | 60130 | All | English |
数据及文献
Data
(A) Flow cyometry analysis of peritoneal macrophages from normal (+/+) and SHIP knockout (-/-) mice. Cells were fixed, permeabilized, and labeled with an isotype control antibody (red) or Anti-Ym1 Antibody, Polyclonal, followed by an Alexa Fluor® 488-conjugated anti-rabbit antibody (blue).
(B) Monocytes isolated from bone marrow of normal and SHIP knockout mice were labeled with Anti-Ym1 Antibody, Polyclonal, followed by an Alexa Fluor® 488-conjugated anti-rabbit antibody.
(C) Western blot analysis of cell lysates from mouse peritoneal macrophages isolated from normal and SHIP knockout mice and probed with Anti-Ym1 Antibody, Polyclonal. Ym1 has a predicted molecular mass of ~45 kDa
