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概要
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human CD19 Positive Selection Kit (Catalog #18054), EasySep™ Human Whole Blood CD19 Positive Selection Kit (Catalog #18084), EasySep™ HLA Whole Blood B Cell Positive Selection Kit (Catalog #18184HLA); partial blocking may be observed, as well as EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood (Catalog #19954HLA) and EasySep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood (Catalog #19961HLA).
技术资料
数据及文献
Data
Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, Alexa Fluor® 488 and anti-human CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, Alexa Fluor® 488. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling with a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood and labeled with Anti-Human CD19 Antibody, Clone HIB19, APC. Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
Figure 3. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).
Figure 4. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ Human Whole Blood CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).
Figure 5. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, PE and antihuman CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD19 Antibody, Clone HIB19, PE. Histograms show labeling of the PBMCs (Start) and isolated cells (Isolated). Labeling with a mouse IgG1, kappa PE isotype control antibody is shown in the bottom panel (open histogram).
Figure 6. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram) or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 7. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD19 Antibody, Clone HIB19, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa PerCP-Cy5.5 isotype control antibody (solid line histogram).
